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. 1999 Nov 15;147(4):775–790. doi: 10.1083/jcb.147.4.775

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© 1999 The Rockefeller University Press

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graphic file with name JCB9907019.f4a.jpg — Steady-state distribution of MHC class II molecules after fractionation of wt and CatS^−/−DC on a 27% Percoll gradient. DC were continuously labeled for 5 h, homogenized, and PNS were fractionated over a 27% Percoll gradient. 1-ml fractions were collected from the bottom of the tube and analyzed by immunoprecipitation, followed by boiling of the samples, 12.5% SDS-PAGE, and autoradiography. (A) Immunoprecipitation for MHC class II dimers from DC from wt and CatS^−/− mice, using the N22 antibody (α/β: MHC class II α/β chain; Ii: invariant chain; p22, p18, p10: breakdown intermediates of Ii of the estimated molecular weight indicated). (B) Same subcellular fractions immunoprecipitated for MHC class I with the p8 antiserum (top and middle panels) (HC, class I heavy chain; β₂m, β-2 microglobulin). (Lower panel) Identical experiment performed with DC from Ii^−/− mice and immunoprecipitated with N22.

graphic file with name JCB9907019.f4b.jpg — Steady-state distribution of MHC class II molecules after fractionation of wt and CatS^−/−DC on a 27% Percoll gradient. DC were continuously labeled for 5 h, homogenized, and PNS were fractionated over a 27% Percoll gradient. 1-ml fractions were collected from the bottom of the tube and analyzed by immunoprecipitation, followed by boiling of the samples, 12.5% SDS-PAGE, and autoradiography. (A) Immunoprecipitation for MHC class II dimers from DC from wt and CatS^−/− mice, using the N22 antibody (α/β: MHC class II α/β chain; Ii: invariant chain; p22, p18, p10: breakdown intermediates of Ii of the estimated molecular weight indicated). (B) Same subcellular fractions immunoprecipitated for MHC class I with the p8 antiserum (top and middle panels) (HC, class I heavy chain; β₂m, β-2 microglobulin). (Lower panel) Identical experiment performed with DC from Ii^−/− mice and immunoprecipitated with N22.