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. 1999 Nov 15;147(4):729–742. doi: 10.1083/jcb.147.4.729

Figure 7.

Figure 7

Genetic and biochemical interactions between Sec34p and Sec35p. a, The sec34-2 and sec35-1 alleles display a synthetic lethal interaction. A diploid strain resulting from the mating of GWY95 (sec34-2) to GWY93 (sec35-1) was sporulated, dissected, and incubated on YPD at 30°C for three days. Ten representative tetrads are shown. b, Interaction of Sec34p and Sec35p by two-hybrid analysis. A two-hybrid strain (PJ69-4A) was transformed with plasmids expressing fusion proteins with the Gal4p-BD (either Gal4p-BD-Sec34p, pSV37; Gal4p-BD-Sec35p, pSV34; or Gal4p-BD-p53, pVA3) and the Gal4p-AD (either Gal4p-AD-Sec34p, pSV35; Gal4p-AD-Sec35p, pSV36; or Gal4p-AD-large T-antigen, pTD3) or with plasmids expressing the BD or AD alone (Gal4p-BD, pGAD424 and Gal4p-AD, pAS2, indicated by --), as shown on the left of the figure. Strains were grown to stationary phase in SC-Trp-Leu and analyzed as described in Fig. 3 with the following exceptions. The diluted samples were spotted onto either SC lacking adenine, leucine, and tryptophan (SC-Ade), or SC lacking histidine, leucine, and tryptophan, but containing 2.5 mM 3-aminotriazole (SC-His), and the plates were incubated at 30°C for three days.