Table 1.
Plasmids Used in this Work
| Plasmid | Description | Source |
|---|---|---|
| pJG103 | 2μm SEC22 URA3 | S. Ferro-Novick (Yale University, New Haven, CT) |
| pSFN2d | 2μm BET1 URA3 | S. Ferro-Novick |
| pNB167 | 2μm YPT1 URA3 | S. Ferro-Novick |
| pGR3 | 2μm BET3 URA3 | S. Ferro-Novick |
| pSED5 | 2μm SED5 URA3 | H. Pelham (MRC, Cambridge, UK) |
| pNB142 | 2μm SEC4 URA3 | P. Novick (Yale University, New Haven, CT) |
| pSK47 | 2μm USO1 URA3 | This laboratory |
| pSK60 | 2μm YKT6 URA3 | This laboratory |
| pSK101 | 2μm BOS1 URA3 | This laboratory |
| pSV11 | CEN SLY1-20 URA3 | This laboratory |
| pSV17 | 2μm SEC35 URA3 | This laboratory |
| pVA3 | 2μm Gal4p-BD-p53 TRP1 | Clonetech, Inc. |
| pTD1 | 2μm Gal4p-AD-T-antigen LEU2 | Clonetech, Inc. |
| pB4 | CEN SEC34 URA3 (library plasmid) | This study |
| pA10 | CEN SEC34 URA3 (library plasmid) | This study |
| pSV22 | CEN SEC34 URA3 | This study |
| pSV24 | YIp SEC34 LEU2 | This study |
| pSV25 | 2μm SEC34 URA3 | This study |
| pSV27 | YIp sec34Δ-construct LEU2 | This study |
| pSV28 | GST-Sec34p | This study |
| pSV30 | His6-Sec34p | This study |
| pSV34 | 2μm Gal4p-BD-Sec35p TRP1 | This study |
| pSV35 | 2μm Gal4p-AD-Sec34p LEU2 | This study |
| pSV36 | 2μm Gal4p-AD-Sec35p LEU2 | This study |
| pSV37 | 2μm Gal4p-BD-Sec34p TRP1 | This study |
| pSOU7 | 2μm RUD3 URA3 (library plasmid) | This study |
| pSK81 | 2μm RUD3 URA3 | This study |