Table 2.
Strains Used in this Work
| Strain | Genotype | Source |
|---|---|---|
| RSY255 | MATa ura3-52 leu2-3,-112 | R. Schekman (University of California at |
| Berkeley, Berkeley, CA) | ||
| RSY942 | MATa sec22-3 ura3-52 lys2-801 | R. Schekman |
| RSY944 | MATa bet1-1 ura3-52 lys2-801 | R. Schekman |
| RSY976 | MATa ypt1-3 ura3-52 | R. Schekman |
| RSY1074 | MATα sly1ts ura3-1 leu2-3,-112 trp1 ade2-1 | R. Schekman |
| RSY1157 | MATa pep4::HIS3 prb::HisG prc::HisG ura3-1 leu2-3,112 trp1-1 ade2-1 can1-100 | |
| SFNY314 | MATa bet3-1 ura3-52 leu2-3,-112 | S. Ferro-Novick (Yale University, |
| New Haven, CT) | ||
| PJ69-4A | MATa ura3-52 leu2-3,112 trp1-901 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 | P. James (University of |
| GAL2-ADE2 met2::GAL7-lacZ | Wisconsin, Madison, WI) | |
| GWY30 | MATa/α ura3-52/ura3-52 leu2Δ1/leu2Δ1 trp1Δ63/trp1Δ63 | This laboratory |
| GWY67 | MATα uso1-1 ura3-52 leu2-3,-112 | This laboratory |
| GWY93 | MATα sec35-1 ura3-52 leu2-3,-112 | This laboratory |
| GWY95 | MATa sec34-2 ura3-52 leu2-3,-112 lys2-801 | This laboratory |
| GWY127 | MATa/α sec34Δ::LEU2/SEC34 ura3-52/ura3-52 leu2Δ1/leu2Δ1 trp1Δ63/trp1Δ63 | This study |
| GWY132 | MATα sec34Δ::LEU2 ura3-52 leu2Δ1 trp1Δ63 +pSV11 (pSLY1-20 CEN URA3) | This study |
| GWY138 | MATa sec32-1 (bos1ts) ura3-52 his4-612 lys2-801 | This laboratory |