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. 1999 Nov 15;147(4):761–774. doi: 10.1083/jcb.147.4.761

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Peroxisomal protein import in PBD054 cells is restored by expression of PEX10 or PEX12. PBD054 cells were transfected with pcDNA3 (A and D), pcDNA3-PEX10 (B and E), or pcDNA3-PEX12 (C and F). 2 d after transfection, the cells were fixed, permeabilized with Triton X-100, and processed for double indirect immunofluorescence using anticatalase antibodies (A–C) and anti–PMP70 antibodies (D–F). Bar, 25 μm.