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Top, Morphology of embryonic structures isolated from E9.5 mouse embryos after pancreatin digestion. Bottom, RT-PCR revealed the medial markers MyoD and Pax3 were expressed in dissected somites (different ratio of Pax3 to MyoD in different lanes depends upon isolation of somites at different cranio–caudal level); Pax3, but not MyoD, in the neural tube; and none of the markers was detectable in dissected dorsal aorta.
