Table 1.
Expression of Different Markers by Aorta-derived and Other Mesodermal Cells
| Cell type: | Aorta | Satellite | Fetal myoblast | Fibroblast |
|---|---|---|---|---|
| MyoD | + | + | + | − |
| Myf-5 | ± | ± | ± | − |
| Desmin | + | + | + | − |
| Smooth α-actin | + | + | + | − |
| MNF | + | + | + | − |
| c-Met | + | + | + | − |
| M-Cad | + | + | + | − |
| VE-Cad | + | + | − | − |
| VEGF-R2 | + | + | + | − |
| P-Selectin (CD62P) | + | + | − | − |
| αM-Integrin (CD11b) | + | + | −/+ | − |
| β3-Integrin (CD61) | + | + | − | − |
| PECAM (CD31) | −/+ | −/+ | −/+ | − |
| von Willebrand | − | − | ND | − |
| CD34 | +/− | +/− | − | − |
Aorta-cells were cloned from seven day-old organ cultures of dorsal aorta from E9.5 day embryos. Immunofluorescence was carried out on at least ten individual clones of each cell type in three separate cultures. Satellite cells were cloned from hind limb skeletal muscles of P10 mice. Fetal myoblasts and fibroblasts were cultured as described ( Cossu et al. 1987). Myoblasts were identified by coexpression of MyoD or desmin.
+, Indicates that >95% of the cells in the clone expressed a high level of the antigen (examples shown in Fig. 3) during the phase of clonal growth. ±, Indicates that ∼10–20% of the cells in most clones expressed Myf-5, revealed by β-galactosidase staining of clones derived from Myf-5/nLacZ embryos. −/+, Indicates that virtually all the cells of a clone expressed high levels of the antigen only after fusion. +/−, Indicates expression of the antigen only during the first days of clonal growth.