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Irreversible growth inhibition of EJ-p73α and EJ-p73β cells after induction of p73α or p73β. EJ-p73α or EJ-p73β cells were seeded directly in (+) tet or (−) tet at 100 cells per 60-mm plate for indicated time period. After readdition of tet for another 2 wk, colonies visualized by Giemsa staining were counted and plotted. The percentage of recoverable colonies for each condition was normalized to the number in (+) tet. All time points were performed in triplicate.
