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Comparison of wild-type and ΔN-hMDM2 interactions with p73α or p73β. 293T cells were transfected with HA-tagged p73α or p73β, together with pcDNA3 vector, hMDM2, or ΔN-hMDM2 as indicated at the top of each lane. Western blot analysis was performed either directly with total lysates (A) or after immunoprecipitation with antibodies as indicated (B).
