Figure 7.

Wild-type but not NH₂-terminal deleted hMDM2 inhibits transcriptional activities of p73α and p73β. H1299 cells in 60-mm plates were cotransfected with the plasmids indicated, as well as the p21 genomic promoter fused to a luciferase reporter and a β-galactosidase expression vector. At 48 h after transfection, luciferase activity was measured and the relative units were plotted. Results of one representative experiment of three independently performed assays are shown. Determinations were made in duplicate for each experimental point.