Figure 8.

p73α and p73β proteins are not stabilized by DNA damage. (A) EJ-p53, EJ-p73α, and EJ-p73β cells were seeded in medium containing indicated tet concentrations. After 8 h, cells were treated with DNA damaging agents as indicated at the top of each lane. 24 h later, lysates were prepared and subjected to SDS-PAGE. Western blot analysis was performed using anti-p53 and HA antibodies. (B) MCF7 cells were treated with DNA damaging agents as indicated at the top of each lane. 24 h later, lysates were subjected to SDS-PAGE. Western blots were performed using anti-p53 or p73 antibodies. EJ-p73α and EJ-p73β in (−) tet were used as positive controls.