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. 1999 Jul 26;146(2):345–360. doi: 10.1083/jcb.146.2.345

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Internalized Tac-furin does not enter the endocytic recycling pathway. (A–C) TRVb-1/TTF cells were incubated for 5 min with FITC–anti-Tac (green) and Cy3-transferrin (red). Cells were chased for 5 (A), 10 (B), or 40 min (C) with Cy3-transferrin before fixation. Single confocal slices are shown. Bars, 10 μm. (D) Cells were incubated with FITC–anti-Tac for 10 min, then chased in the absence (dashed line) or presence (solid line) of antifluorescein polyclonal antibodies for the indicated times before fixation and epifluorescence imaging. Fluorescence intensities per cell were calculated as described in Materials and Methods. Means ± SEM for 10 fields of cells in a single experiment are shown.

Internalized Tac-furin does not enter the endocytic recycling pathway. (A–C) TRVb-1/TTF cells were incubated for 5 min with FITC–anti-Tac (green) and Cy3-transferrin (red). Cells were chased for 5 (A), 10 (B), or 40 min (C) with Cy3-transferrin before fixation. Single confocal slices are shown. Bars, 10 μm. (D) Cells were incubated with FITC–anti-Tac for 10 min, then chased in the absence (dashed line) or presence (solid line) of antifluorescein polyclonal antibodies for the indicated times before fixation and epifluorescence imaging. Fluorescence intensities per cell were calculated as described in Materials and Methods. Means ± SEM for 10 fields of cells in a single experiment are shown.