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. 1999 Jul 26;146(2):345–360. doi: 10.1083/jcb.146.2.345

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Wortmannin inhibits the delivery of internalized Tac-furin to the TGN, but has a milder effect on Tac-TGN38 transport. TRVb-1/Tac-furin (A–D) and Tac-TGN38 (E and F) cells were pretreated with 100 nM wortmannin as described in Materials and Methods. Cells then were incubated for 15 min with A488–anti-Tac (C) plus DiI-LDL (D) or Cy3–anti-Tac alone (A and E), followed by a 45-min chase, maintaining the concentration of wortmannin throughout. After fixation, cells that received Cy3–anti-Tac were stained with NBD-C₆-ceramide (Cer; B and F). Z-series confocal projections are shown. Arrows in C and D indicate some points of colocalization to assist the reader. Bars, 10 μm.