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. 1999 Jul 26;146(2):405–414. doi: 10.1083/jcb.146.2.405

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Line-scan analysis of global Ca²⁺ spikes induced by ACh or caged IP₃. (A, C, and E) Increases in [Ca²⁺]_i recorded with the line-scan mode of the confocal microscope and with fluo-3 as the Ca²⁺ indicator. The trigger zone and basal area of the acinar cells are denoted by T and B, respectively. The cells were stimulated either with 10 μM ACh (A), or by rapid (C) or slow (E) photolysis (indicated by tracings above images) of 100 μM caged IP₃. (B, D, and F) Time courses of [Ca²⁺]_i in the trigger zones and basal areas for the cells shown in A, C, and E, respectively.