Table 2.
Effects of Inhibitors on Shc- or FAK-induced Migration
| Transfectant | Inhibitor | Migration rate ± SD | Increase vs. PTEN | Percentage inhibition |
|---|---|---|---|---|
| μm/3 h | μm/3 h | |||
| PTEN | None | 54 ± 17 | — | — |
| PTEN + Shc | None | 110 ± 24 | 56 | 0% |
| PTEN + Shc | PD98059 | 56 ± 18 | 2 | 96% |
| PTEN + Shc | Wortmannin | 99 ± 18 | 43 | 23% |
| PTEN + FAK | None | 117 ± 24 | 63 | 0% |
| PTEN + FAK | PD98059 | 111 ± 15 | 57 | 10% |
| PTEN + FAK | Wortmannin | 76 ± 65 | 22 | 65% |
U-87MG cells, transiently transfected with the indicated expression plasmids, were treated with inhibitors or left untreated, and then were analyzed for rates of cell migration as described in Materials and Methods. The column entitled Increase shows net increases in the migration rate compared to the PTEN-reconstituted control.