Table 2.
Monocytes associated with loss of: | |||
---|---|---|---|
Monocytes in group | VE-Cadherin | β-Catenin | Plakoglobin |
n | % | % | % |
>5 | 1 ± 2 | 2 ± 3 | 0 ± 0 |
3–5 | 15 ± 13 | 31 ± 3 | 37 ± 5 |
1 or 2 | 83 ± 14 | 69 ± 6 | 64 ± 5 |
Flow adhesion assays using PBMC were performed as detailed in Materials and Methods. HUVEC monolayers were recovered and stained for components of the VE-cadherin complex. Monocytes associated with loss of VE-cadherin, β-catenin, or plakoglobin were scored as to whether they were in groups of: >5; 3–5; or 1 or 2 monocytes. The results are expressed as the percentage of monocytes in each group associated with loss of junctional staining (mean ± SD).