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. 2000 Jan 10;148(1):45–58. doi: 10.1083/jcb.148.1.45

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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3D reconstruction of GPCs. Cells were treated as in Fig. 4. VSVG-labeled structures in 50-nm serial sections were photographed and digitized for 3D reconstruction. a–h show eight consecutive serial sections of the GPC of interest. i and j depict the surface of the GPC (green) and its relationship to the PM (red) at two different angles. Although in each single section the GPC appeared as a tubule (arrows), 3D reconstruction revealed the GPC as a flattened sacculus positioned parallel to the PM. Bar: (a–h) 500 nm; and (i–j) 200 nm.