Figure 1.

In situ hybridization for GLUT3 mRNA. Bright-field (Upper) and dark-field (Lower) views of sections of wt Hepa-1, c4, Rc4, and c31 xenografts. Regions of high-intensity signal are seen in the dark-field views of wt Hepa-1, Rc4, and c31 tumors, but not in the c4 tumors. Each of the four views includes areas of necrosis. Arrows within necrotic areas in the bright-field views point toward the boundary with viable tumor cells. In the wt, Rc4, and c31 tumors regions of high-intensity signal border on these areas of necrosis. Final magnification, ×80.