FIGURE 4.

Intermolecular interfaces of the FYVE-DPC and PX-DPC complexes. The intermolecular hydrogen bonds present in the lowest energy structure of FYVE-DPC and PX-DPC complexes are shown in A and C, and the intermolecular interactions present in >30% of the best 20 structures are shown in B and D. The three-dimensional images depict proteins as gray ribbons, hydrogen-bonding residues as gray sticks, Ins(1,3)P₂ in red, micelles in blue, and intermolecular hydrogen bonds as green dotted lines. The two-dimensional representations show DPC and Ins(1,3)P₂ chemical structures together with the consensus intermolecular contacts to different protein residues (see also Supplementary Tables S1 and S2). Hydrogen bonds and salt bridges are drawn as green dotted lines, and the intermolecular hydrophobic contacts as black horizontal double lines.