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. 2007 Sep 21;94(2):515–524. doi: 10.1529/biophysj.107.115923

FIGURE 5.

FIGURE 5

Chemical shift changes of Vam7p PX amides upon DPC/CHAPS (3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonic acid) titration. A shows three superimposed [1H,15N]HSQC spectra of Vam7p PX (65 μM) collected at 298 K on a Varian (Palo Alto, CA) INOVA 800 MHz spectrometer during titration with mixed DPC and CHAPS micelles (50 mM phosphate buffer, pH 7.0, 100 mM KCl, 1 mM Tris-(2-carboxyethyl) phosphine, and 1 mM NaN3). The histogram from B displays the normalized chemical shift changes (51) induced by the protein-micelle interactions. Residues with significant chemical shift changes are labeled.