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Effects of troponin I mutations on binding of S1 to actin in the presence of ATP. Mean values of the fraction of S1-ATP bound with different actin concentrations at 0.04 M ionic strength are shown ± SD (n = 3). Binding conditions are: 25°C, pH 7, 0.1 μM S1, 3 mM ATP, 10 mM MOPS, 5 mM MgCl2, 14 mM NaCl, 1 mM dithiothreitol, 0.5 mM CaCl2. There was no significant difference in binding between the wild-type and mutants at the actin concentrations measured.
