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. 2007 Sep 14;94(2):469–479. doi: 10.1529/biophysj.107.109470

FIGURE 7.

FIGURE 7

Comparison of SLDT and ELDT. (A) Examples of exponential diffusion profiles obtained in WT and connexin-expressing C6 cells after scrape loading (n = 10 for each curve). (B) Diffusion profiles obtained with electroporation loading (n = 8 for each curve). The shaded curves are copies of the curves illustrated in A, added for comparison. (C and D) Summary graphs of the spatial constant of dye spread λ determined in the experiments illustrated in A and B, respectively. Stars give significancies as compared with C6-WT (n = 10 and 8, respectively). (E) Increase of λ in connexin-expressing C6 cells as compared with WT with the two loading protocols. Dye spread was more than twice as large in ELDT as compared with SLDT experiments. Stars show significances as compared with SLDT. (F) Decrease of λ determined with ELDT in cultures that received an SLDT scratch before electroporation. The picture illustrates the horizontal ELDT strip and the vertical SLDT scratch applied just before electroporation. Dye spread decreased to the level observed in SLDT when ELDT was done in cultures that received the SLDT scratch. Stars indicate significances as compared with ELDT (n = 20).