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. 1997 Jul 22;94(15):8173–8178. doi: 10.1073/pnas.94.15.8173

Figure 2.

Figure 2

Transgenic human AChE (hAChE) mRNA expression induces a transient embryonic enhancement in ChAT mRNA levels. (A) RT-PCR analyses. RNA extracted from spinal cord of control (C) and transgenic (T) mice at the noted ages was subjected to kinetic RT-PCR using primers for the noted mRNAs (see Experimental Procedures). Aliquots of amplified DNA representing human (h) or mouse (m) AChE or ChAT mRNAs were removed every third cycle from cycle 21, representing differences of ca. 8-fold between samples. Products were electrophoresed and stained with ethidium bromide. Note that hAChE mRNA is present only in transgenic mice, that endogenous AChE mRNA levels are similar in control and transgenic animals, and that while mChAT levels are undetectable in control E17 embryos, a prominent signal, marked by an asterisk, is observed in transgenic embryos. In postnatal mice, ChAT mRNA levels in transgenic and control spinal cord are indistinguishable. NB, newborn. (B) In situ hybridization. Seven-micrometer sections from the lumbar spinal cord of control (C) and transgenic (T) E17 embryos were subjected to in situ hybridization. Fast-red (Boehringer Mannheim) served for detection. Note the intense AChE and ChAT mRNA signals in transgenic cell bodies.