Figure 3.

Neurexin Iβ mRNA is dramatically suppressed in embryonic and newborn transgenic mice. Primers specific for neurexin Iβ and IIIβ mRNAs (see Experimental Procedures) were used in semiquantitative RT-PCR on spinal cord RNA from control (C) and transgenic (T) mice at the noted ages. Note the dramatic reduction in signal intensities for neurexin Iβ in embryonic (E17) and newborn (NB) transgenic mice compared with controls and to 5-week-old (5 Wk) mice. Primers for β-actin mRNA served to verify quantity and quality of RNA in each sample. Shown are PCR products sampled every third cycle (i.e., reflecting 8-fold differences) from cycle 21 for β-actin and cycle 24 for neurexins.