Figure 2.

ShcA and p66^ShcA localization in mouse sagittal sections. ShcA (A, D, and G) and p66^ShcA (B, E, and H) expression in mouse sagittal sections. Anterior is to the left. (A–C) E10.5, (D–F) E12, (G–I) P2. Bright-field views of sections counterstained with cresyl violet are also shown (C, F, and I). Both ShcA and p66^ShcA showed developmentally regulated hybridization signal exclusively in the brain. Their patterns of expression appeared to be temporally regulated and practically coincide with the proliferative epithelium (ventricular zone, VZ) all throughout development (arrowheads). p66^ShcA mRNA was expressed in all territories showing the ShcA signal, although to a lesser extent as expected from the relative levels of the three isoforms. Postmitotic regions (arrows) showed no specific hybridization signal. On the contrary, outside the brain Shc and p66^shcA expression patterns appear to be both ubiquitous and homogenous (asterisks in D–F). Sense probes gave no specific hybridization signals (data not shown). Cx, cortex; Mes, mesencephalon; My, myelencephalon; Rh, rhombencephalon; Te, telencephalon.