Figure 4.

Agonistic effects of the imidates DMS and MAI with rat muscle nAChR expressed in Xenopus oocytes. (A) Whole-cell current (rat muscle nAChR) induced by DMS application (upper trace) and by MAI application (lower trace). Whole-cell current induced by ACh application is shown before and after DMS/MAI application. (B) Dose–response curves of DMS (□) and MAI (•). The relative amplitudes are plotted against the DMS/MAI concentration in a double-logarithmic plot. The amplitudes are normalized to the response induced by 0.1 μM ACh. (C) Competition of DMS (•) and MAI (▪) with [³H]ACh at nAChR-rich membranes from Torpedo californica. Bound [³H]ACh in the presence of DMS/MAI is normalized to bound [³H]ACh in the absence of DMS/MAI and plotted against the concentration of effector (DMS/MAI).