Figure 2.

Binding of opioid receptor ligands. Saturation binding experiments, with either the nonselective opioid receptor antagonist [³H]naloxone (A) or the μ-selective agonist [³H]DAMGO (B) to membranes prepared from HEK 293 cells expressing the C. commersoni μ-opioid receptor, were carried out as described. Specific binding was calculated by subtracting the nonspecific binding (determined in the presence of 10 μM naloxone) from the total binding determined using a range of concentrations of either [N-allyl-2,3-³H]naloxone (0.1–30 nM) or [³H]DAMGO (0.1–30 nM). (Insets) Scatchard plots calculated from the binding data shown.