Figure 3.

Displacement of [³H]naloxone binding by DAMGO but not by either DPDPE or U50488. The binding of 10 nM [N-allyl-2,3-³H]naloxone to membranes prepared from HEK 293 cells expressing the C. commersoni μ-opioid receptor was determined in the presence of a range of concentrations of the μ-selective agonist DAMGO (0.1 nM to 10 μM; A), the δ-selective agonist DPDPE (0.1 nM to 10 μM; B), and the κ-selective agonist U50488 (0.1 nM to 3 μM; C).