Figure 4.

Effects of nNOS antisense treatment on systemic morphine analgesia in naive mice. Three doses of saline or the indicated oligodeoxynucleotide (5 μg) were given on days 1, 3, and 5 either (a) i.t. or (b) i.c.v. Four hours after the last oligodeoxynucleotide treatment mice were tested with morphine (5 mg/kg, s.c.). Antisense C given either i.c.v. or i.t. lowered morphine analgesia significantly (P < 0.001) compared with saline controls. No significant changes were seen with any other antisense or any of the mismatch controls.