Figure 6.

Effects of supraspinal and spinal nNOS antisense on morphine tolerance. Groups of mice (n ≥ 20) received three doses of the indicated oligodeoxynucleotide (5 μg) (a) i.c.v. or (b) i.t. every other day. Starting on the day of the last oligodeoxynucleotide treatment (day 1), the mice received daily morphine injections (5 mg/kg, s.c.). The analgesic activity is given on day 1 and day 5. The analgesic activity in the control and mismatch groups on day 5 are all significantly different (P < 0.001) from their own values on day 1. The responses of the antisense treated animals on day 5 do not differ from their values on day 1, but they do differ from the day 5 values of the control and mismatch groups (P < 0.002).