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Inhibition of the Δ1-pyrroline-5-carboxylate synthetase (P5CS) activity of GK/GPR products of the tomPRO1 clone. P5CS activity was assayed in crude extracts of E. coli strains carrying the tomPRO1–1 clone in pKSII+. Assays were carried out in the presence of the indicated concentrations of proline, as described in Materials and Methods. Results are expressed as percent of specific activities in the absence of proline, which was 13 nmol (min)−1⋅(mg total protein)−1.
