Figure 3.

Northern blot and nuclear run-off transcription analyses of 1/2 N gene low expressor (8-2) and high expressor (8-127) lines. (A) Northern blot analysis of 8-2 and 8-127 R₁ plants. Total RNAs were isolated from transgenic plants (15 μg per lane) and analyzed by Northern blotting using as the probe the N gene of TSWV-BL. Lanes 1–6, R₁ plants of line 8-2; lanes 7–12, R₁ plants of line 8-127; lane 13, a nontransgenic control. Size of 1/2 N gene transcript is ≈670 bp. (B) Nuclear run-off transcription analysis on R₁ plants. Labeled nuclear RNAs were hybridized to restriction enzyme-digested N gene, nptII, and actin fragments separated on agarose gels and blotted onto membranes. The nuclei used in the assays were isolated from an 8-2 R₁ plant and an 8-127 R₁ plant.