Figure 4.

Comparison of transcript accumulations in plants expressing N gene segments of different lengths that had been tested for resistance and post-transcriptional gene silencing. Ten micrograms per lane of total RNAs isolated from transgenic plants was used in Northern blots which were probed with the N gene of TSWV-BL. Lanes 1 and 2, two nonsilenced, virus-susceptible R₁ plants with the full-length N gene; lane 3, a silenced, virus-resistant R₁ plant with 2/2 N gene; lane 4, a nonsilenced, virus-susceptible R₁ plant with 2/2 N gene; lane 5, a nontransformed control; lanes 6–9, four nonsilenced, virus-susceptible R₁ plants with 3/4 N gene; lanes 10–13, four nonsilenced, virus-susceptible R₁ plants with 5/8 N gene. Sizes of transcripts for full-length N gene, 2/2 N gene, 3/4 N gene, and 5/8 N gene are ≈1150 bp, ≈730 bp, ≈500 bp, and ≈390 bp, respectively.