Table 1.

Primers used in cloning N gene segments of TSWV and the entire GFP gene

Name	Nucleotide positions*	Sequence
For the N gene fragments
91-84	2766–2744	5′-AGCTAATCTAGAACCATGGATGACTCACTAAGGAAAGCATTGTTGC
93-89	2669–2650	5′-TACAGTTCTAGAACCATGGTCTGGAAAACCTTGACCAG
93-85	2576–2556	5′-TACAGTTCTAGAACCATGGTAAAGCGATTTTACTTTTGGTA
92-55	2373–2354	5′-AGATTCTCTAGACCATGGTGACTTGATGAGCAAAGTCTGTGAGGCTTGC
93-91	2266–2248	5′-TACAGTTCTAGAACCATGGAAAATACAAGGATCTCGGG
93-87	2153–2133	5′-TACAGTTCTAGAACCATGGTAGAAGGGGAAAGAGTATGCTG
90-47	1918–1937	5′-AGCATTGGATCCATGGTTAACACACTAAGCAAGCAC
93-86	2158–2177	5′-TCTTGAGGATCCATGGCTGATCTTCATTCATTTCAA
93-90	2269–2288	5′-TCTTGAGGATCCATGGATCCTGATATATAGCCAAGA
92-53	2383–2402	5′-TACAGTGGATCCATGGTTAAGGTAATCCATAGGCTTGAC
93-84	2577–2597	5′-TCTTGAGGATCCATGGCTTAATAACCTTCATTATGC
93-88	2671–2690	5′-TCTTGAGGATCCATGGAAAAGTCTTGAAGTTGAATG
94-265	2556–2530	5′-AGCTAATCTAGAACCATGGATGAAAAATTACCATAAAGAAAACTTCAGAC
94-266	2182–2206	5′-AGCATTGGATCCATGGTTAGTTACCTAGTTTTCTTTTCAGCACAGTGCAAACT
For the GFP gene
5′ GFP	NA	5′-TGAACATCTAGAACCATGGGTAAAGGAGAAGAACTTTTCACTGG
3′ GFP	NA	5′-TGAACAGGATCCATGGTCTACGAATGCTATTATTTGTATAGTTC

NA, not applicable. 

^*Nucleotide position number of each primer for the N gene segments was as published by Pang et al. (33). The N gene and GFP gene sequences are underlined and the rest of the sequences correspond to the primers used for cloning. Out-of-frame ATG and stop codons for the untranslatable versions in the 5′ primers are shown in boldface.