Figure 2.

(A) Diffraction quality crystals of rat liver F₁. (B) Scan of an SDS/PAGE gel of redissolved F₁ crystals. Crystals of F₁ were dissolved in 50 μl of 250 mM KP_i plus 5 mM EDTA (pH 7.5), and 15 μg were subjected to SDS/PAGE in cylindrical gels. (C) Capacity of redissolved crystals to rebind to F₁-depleted inner membrane vesicles of mitochondria and regain sensitivity to oligomycin, an inhibitor of oxidative phosphorylation. Where indicated, 1 μg oligomycin was present. (D) Relative capacity of ATP and MgCl₂ to initiate the F₁ ATPase reaction. The decrease in optical density of NADH at 340 nm was monitored. Reaction 1 was initiated with 4 mM ATP, and reaction 2 was initiated with 4.8 mM MgCl₂.