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. 1998 Sep 15;95(19):11083–11088. doi: 10.1073/pnas.95.19.11083

Figure 2.

Figure 2

The effects of Adiol on the growth of yeast cells and on the AR transcriptional activity in human prostate cancer cells. (A) Yeast strain, w3031b, transformed with either pG1-wtAR or pG1-mtAR708 and reporter plasmid (pARE-his3) were cultured in -3SD medium (-histidine, -leucine, and -tryptophan) with 25 mM 3-aminotriazole in the absence or presence of hormones as indicated. When the OD660 value (X) was between 0.6–1.2, the cell density (Y × 107 cells/ml) was calculated by the formula: Y = 0.276 × 100.826x. Relative cell number represents the means ± SD of three independent experiments. (B) DU145, PC-3, or LNCaP cell line was transfected with MMTV-CAT (3.5 μg) and with (for DU145 and PC-3) or without (for LNCaP) wtAR expression plasmid (1.5 μg). Twenty-four hours after transfection, cells were cultured an additional 24 h in the absence or presence of 1 nM DHT or increasing concentrations of Adiol. The CAT activity was determined and the mock treatment was set as 1-fold. Values are the means ± SD of at least three determinations.