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Activation of AR by Adiol in the presence of an AR coactivator, ARA70, in DU145 cells. DU145 cells first cotransfected with 3.5 μg reporter gene and 1.5 μg AR expression plasmid (wtAR, mtAR877, or mtAR708) with or without 4.5 μg ARA70, were treated with increasing concentrations of Adiol, DHEA, 7-oxo-DHEA, or T. The induction ratios relative to control (mock) are the means ± SD of at least three determinations.
