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Dose-dependent physical interaction between AR and ARA70 in the presence of Adiol by mammalian two-hybrid assay. DU145 cells were cotransfected with 3.5 μg pGAL0-wtAR (A), 3.5 μg pGAL0-mtAR877 (B), or 3.5 μg pGAL0-mtAR708 (C), 3.5 μg pVP16-ARA70, and 2.5 μg CAT reporter gene, and treated 24 h with Adiol, DHEA, or 7-oxo-DHEA. Data represent the means ± SD of at least three determinations.
