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CstF-64 protein levels increase specifically after serum stimulation. 3T6 fibroblasts were starved and then stimulated with serum for 18 hr as described in the legend to Fig. 1. Protein samples from the 18-hr serum-treated cells were diluted and run on SDS/PAGE along-side the starved cell sample. The blot was probed with antibodies to CstF-64, BAX, or GAPDH.
