Figure 3.

CstF complex shows increased CstF-64 subunit association in growing cells. 3T6 cells were lysed and incubated with mouse monoclonal anti-CstF-50 or anti-human CD8 antibody as described in Materials and Methods. The immunoprecipitated proteins in the CstF complexes were separated by SDS/PAGE, blotted to a membrane and probed sequentially with anti-CstF-64 and CstF-77 antibodies. The proteins were visualized as described in Materials and Methods.