Table 1.

Stimulation of primary splenic human B-cells induces proliferation and CstF-64 protein

Treatment	Amounts of polyadenylation/cleavage factors*		Proliferation [3H]TdR cpm per 106 cells	Secreted IgM protein (μg/ml) per 106 cells	Ratio Ig μ sec:mb mRNA by PCR
	CstF-64	CstF-77, CPSF-30, and CPSF-100
None	1	1	2,000	0	0.8:1
CD40 ligand	5	1	75,000	3	0.8:1
CD40L + IL-10	5	1	90,000	60	2.4:1
IL-10	1	1	10,000	0	0.8:1
SAC	2–3	1	37,000	60	—

Data is representative of three separate stimulations. 

^*Normalized to GAPDH loading control.