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. 1998 Sep 15;95(19):11107–11112. doi: 10.1073/pnas.95.19.11107

Figure 3.

Figure 3

Inhibition of tyrosine phosphorylation of Stat3 by PMA. MM6 cells (A) or CHO and 293T cells (B) were treated with PMA followed by a 12-min stimulation with IL-6. (A) Cell extracts were immunoprecipitated with Stat3 antibody and analyzed by using immunoblotting. (B) Cell extracts were fractionated by SDS/PAGE and analyzed by immunoblotting with antibodies specific for Stat3 phosphorylated on tyrosine-705.