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(A) IFNα activation of Stat3 is not inhibited by PMA. MM6 cells were incubated with PMA for 15 min, followed by a 12-min stimulation with IFNα (8 ng/ml). Cell extracts were assayed for binding to the hSIE oligonucleotide by using EMSA. (B) Inhibition of Stat3S727A mutant. CHO cells were transfected with plasmids encoding Stat3S727A containing a FLAG epitope tag, stimulated with PMA and IL-6 and anti-FLAG immunoprecipitates analyzed by immunoblotting.
