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Inhibition of Jaks by PMA. (A) MM6 cells were treated with PMA followed by a 12-min stimulation with IL-6. Seventy-five percent of immunoprecipitates were analyzed for Jak activity (17) and 25% analyzed by using immunoblotting. (B) CHO cells were treated with PMA followed by stimulation with 100 ng/ml of oncostatin M, and immunoprecipitates were analyzed by immunoblotting.
