Figure 5.
Specificity of reconstituted system for hemimethylated oriC. Gel retardation assays were performed in the presence or absence of SeqA (2.9 nM) (a, c, and e) and/or fraction B (FxB, 40 ng/μl) (b, d, and f), as indicated. The concentrations of hemimethylated (a and b), fully methylated (c and d), or unmethylated (e and f) 32P-labeled oriC were 50 pM (lanes a–d) and 200 pM (lanes e–h). Autoradiograms were exposed for 12 hr (lanes a–d) or 3 hr (lanes e–h). Arrows indicate position of free oriC.