Figure 3.

Xtc1p interacts directly with the activation domains of several different activators. (Upper) Immunoblot analysis of Xtc1p (arrow) in the input yeast extract (lane 1) and high-salt eluates (lanes 2–9) from a series of affinity columns. The column ligand is indicated above each lane. (Lower) Coomassie blue-stained SDS gel showing the input recombinant Xtc1 protein (lane 1) and high-salt eluates (lanes 2–9) from a duplicate set of affinity columns. For both panels, 20% of the input sample and 50% of the column eluate were analyzed.