Table 1.
Frequencies of ORF+ and ORF− clones panned from the native library
| Target | ORF+ | ORF− | Total clones | % ORF− | Frame 0 | Frame +1 | Frame −1 | |
|---|---|---|---|---|---|---|---|---|
| PAb 240 | 12 | 0 | 12 | 0.0 | ||||
| PAb 1620 | 2 | 0 | 2 | 0.0 | ||||
| MAb 3G5 | 3 | 0 | 3 | 0.0 | ||||
| MAb 2C3 | 1 | 0 | 1 | 0.0 | ||||
| Total | 18 | 0 | 18 | 0.0 | ||||
| GHBP | 1 | 1 | 2 | 50.0 | 1 | 0 | 0 | |
| IGF-1R | 11 | 4 | 15 | 26.7 | 0 | 4 | 0 | |
| IL-4R | 5 | 14 | 19 | 73.7 | 2 | 8 | 4 | |
| IL-6R | 1 | 7 | 8 | 87.5 | 1 | 3 | 3 | |
| IL-9R | 6 | 5 | 11 | 45.5 | 0 | 3 | 2 | |
| IL-10R | 6 | 20 | 26 | 76.9 | 4 | 7 | 9 | |
| gp130 | 9 | 16 | 25 | 64.0 | 3 | 4 | 9 | |
| MDM2 | 20 | 7 | 27 | 25.9 | 5 | 1 | 1 | |
| Total | 59 | 74 | 133 | 56% | 21.6% | 40.5% | 37.8% |
A set of identical sequences obtained from panning a given target is counted as one clone. All of the clones were shown by ELISA to produce phage that bind to the target with a signal-to-background ratio >2.