Figure 1.

Nucleic acid and amino acid sequence of CiNR1 and SDS/PAGE of in vitro-synthesized CiNR1 protein. (A) The deduced amino acid sequence begins with a presumptive initiation codon at nucleotide 98 but also contains one downstream Met codon at nucleotide 152, which could be an initiator too. Multiple upstream stop codons (underlined in the 5′ region) suggest the first Met codon as the translation start-site. The ORF contains 587 amino acids and is followed by the 3′ untranslated region of 225 bp, which contains a consensus polyadenylation signal (underlined in the 3′ region). Eight cysteines of the DNA binding domain are in bold. (B) SDS/PAGE analysis of l-[³⁵S]-Methionine-labeled protein encoded by the complete cDNA of CiNR1 in rabbit reticulocyte lisate. The translation products were analyzed in 10% SDS polyacrylamide gels. The control line is the translation product of CiNR1 cDNA antisense strand.