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. 1981 Sep;147(3):962–971. doi: 10.1128/jb.147.3.962-971.1981

Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication.

J Inselburg
PMCID: PMC216135  PMID: 6268615

Abstract

This report describes a method for isolating mutants of plasmid ColE1 that exhibit unstable maintenance and altered replication characteristics. It also describes the initial characterization of four mutants isolated by that method. A chimeric plasmid, pHSG124, containing a ColE1 derivative and a temperature-sensitive replication derivative of pSC101 was mutagenized in vitro, using hydroxylamine. By adjusting the growth conditions of transformants containing the mutagenized chimeric deoxyribonucleic acid, it was possible to rapidly screen colonies and identify those that had a high probability of carrying ColE1 mutants that exhibit unstable maintenance. Of those mutants, some exhibited altered copy number or accumulated catenated structures. Evidence is presented which suggests that the mutations in three of the mutants are probably located in the HaeII A fragment of ColE1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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