Abstract
Deoxyribonucleic acid fragments bearing the gene for diphtheria toxin have been identified in restriction enzyme digests of deoxyribonucleic acids from beta-converting and gamma-nonconverting corynebacteriophages. A combination of physical and genetic evidence has established that the Bam HI band C fragment of beta phage deoxyribonucleic acid, which carries the specific phage attachment site (Buck and Groman, J. Bacteriol. 148:131-142, 1981), also carries most, and probably all, of the gene for diphtheria toxin. A detailed restriction map of this tox-bearing Bam HI fragment has been developed, and the locations and orientation of the tox gene and the attP site within this fragment have been established.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bacha P., Murphy J. R. Isolation and characterization of extragenic suppressor strains of Corynebacterium diphtheriae. J Bacteriol. 1978 Dec;136(3):1135–1142. doi: 10.1128/jb.136.3.1135-1142.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buck G. A., Groman N. B. Genetic elements novel for Corynebacterium diphtheriae: specialized transducing elements and transposons. J Bacteriol. 1981 Oct;148(1):143–152. doi: 10.1128/jb.148.1.143-152.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buck G. A., Groman N. B. Physical mapping of beta-converting and gamma-nonconverting corynebacteriophage genomes. J Bacteriol. 1981 Oct;148(1):131–142. doi: 10.1128/jb.148.1.131-142.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buck G., Groman N., Falkow S. Relationship between beta converting and gamma non-converting corynebacteriophage DNA. Nature. 1978 Feb 16;271(5646):683–685. doi: 10.1038/271683a0. [DOI] [PubMed] [Google Scholar]
- Costa J. J., Michel J. L., Rappuoli R., Murphy J. R. Restriction map of corynebacteriophages beta c and beta vir and physical localization of the diphtheria tox operon. J Bacteriol. 1981 Oct;148(1):124–130. doi: 10.1128/jb.148.1.124-130.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
- GROMAN N. B., EATON M. Genetic factors in Corynebacterium diphtheriae conversion. J Bacteriol. 1955 Dec;70(6):637–640. doi: 10.1128/jb.70.6.637-640.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Groman N., Laird W. Heat-inducible mutants of corynebacteriophage. J Virol. 1977 Sep;23(3):587–591. doi: 10.1128/jvi.23.3.587-591.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes R. K., Barksdale L. Genetic analysis of tox+ and tox- bacteriophages of Corynebacterium diphtheriae. J Virol. 1969 Jun;3(6):586–598. doi: 10.1128/jvi.3.6.586-598.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes R. K. Characterization and genetic mapping of nontoxinogenic (tox) mutants of corynebacteriophage beta. J Virol. 1976 Jul;19(1):195–207. doi: 10.1128/jvi.19.1.195-207.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kanei C., Uchida T., Yoneda M. Isolation from corynebacterium diphtheriae C7(beta) of bacterial mutants that produce toxin in medium with excess iron. Infect Immun. 1977 Oct;18(1):203–209. doi: 10.1128/iai.18.1.203-209.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laird W., Groman N. Isolation and characterization of tox mutants of corynebacteriophage beta. J Virol. 1976 Jul;19(1):220–227. doi: 10.1128/jvi.19.1.220-227.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laird W., Groman N. Orientation of the tox gene in the prophage of corynebacteriophage beta. J Virol. 1976 Jul;19(1):228–231. doi: 10.1128/jvi.19.1.228-231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laird W., Groman N. Prophage map of converting corynebacteriophage beta. J Virol. 1976 Jul;19(1):208–219. doi: 10.1128/jvi.19.1.208-219.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy J. R., Michel J. L., Teng M. Evidence that the regulation of diphtheria toxin production is directed at the level of transcription. J Bacteriol. 1978 Aug;135(2):511–516. doi: 10.1128/jb.135.2.511-516.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pappenheimer A. M., Jr Diphtheria toxin. Annu Rev Biochem. 1977;46:69–94. doi: 10.1146/annurev.bi.46.070177.000441. [DOI] [PubMed] [Google Scholar]
- Thomashow M. F., Nutter R., Montoya A. L., Gordon M. P., Nester E. W. Integration and organization of Ti plasmid sequences in crown gall tumors. Cell. 1980 Mar;19(3):729–739. doi: 10.1016/s0092-8674(80)80049-3. [DOI] [PubMed] [Google Scholar]
- Uchida T., Pappenheimer A. M., Jr, Greany R. Diphtheria toxin and related proteins. I. Isolation and properties of mutant proteins serologically related to diphtheria toxin. J Biol Chem. 1973 Jun 10;248(11):3838–3844. [PubMed] [Google Scholar]